The Deinococcus species are famous for their ability to survive diverse extreme stresses, including gamma radiation, UV light, desiccation and oxidation, which attracts more attention from the scientific researchers. Alternative sigma factors play an important role in regulating genes’ expression, which confers stress resistance to the vegetative cell. Previous study revealed that Sig1 (DR0180), classified into Extra-Cytoplasmic Function (ECF) sigma factors based on sequence similarity, can protect D. radiodurans from heat shock in transcriptional and translational level. In this study, Our aim is to find the activation of Sig1 and its regulons under these harsh conditions and delineate the Sig1-mediated regulatory network.