During the last decades, the agriculture intensification has led to a dramatical increase in pesticides use in the Pampas region (South America). Herbicides are the most applied group of pesticides and their use is expected to continue growing. Despite their benefits on agricultural yields, herbicides could pose serious human health and environmental risks. This PhD project focuses on the ecological effects of mixtures of herbicides on soil functioning, at current field application schemes (i.e. amounts and frequency of applications) for soybean production. Semi-field and field experiments will be conducted to fully assess herbicides impact and support further recommendations for weeds control.