Biological recovery of selenium (Se) for selenium-laden wastewater treatment holds promise as it could potentially provide a stable, sustainable Se supply and eliminate the toxic effects of Se contaminations. This thesis aimed to remove and recover selenium from simulated wastewater, investigating the main objective: to produce highly pure recoverable elemental selenium (Se0) with a relatively high selenate reduction rate. Two pathways to produce crystalline elemental selenium (Se0) for potential selenium recovery were proposed: the recrystallization of amorphous Se0 and the crystalline Se0 production from SeS2 by combining the selenate to selenite process with the sulfate-reducing process.