Advanced Proteomics

Background

Mass spectrometry (MS)-based proteomics is the most comprehensive approach for the quantitative profiling of proteins, their interactions, and modifications. Proteomics is the study of the proteome, the set of proteins expressed in an organism. The proteome can change dramatically depending on developmental stage, biological conditions, and subcellular localisation. Proteomics is able to capture detailed snapshots of the proteome using mass spectrometry. The rapid development in technical advances of instrumentation has led to new bioinformatics approaches. In this course the focus will be on sample preparation, data analysis, and new methods in MS-based proteomics approaches.

Aim

Participants will learn

• how to make a proteomics sample
• perform proteomic data analysis using MaxQuant
• integrate results into biological research.

Target group

PhD candidates or Postdocs interested in this course should work on proteomics projects or plan to use proteomics in the near future and have a strong background in nutrition, biochemistry, physiology, molecular biology or a comparable discipline.